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甲状腺激素对新生期大鼠下丘脑Go蛋白 亚单位的影响 |
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蔡东升 罗敏 陈源 丁伟 陈刚 金群燕 笪翠娣 陈家伦
【摘要】 目的 研究新生期Wistar大鼠下丘脑Go蛋白α亚单位(Goα)的分布及甲状腺激素对其表达的影响。方法 采用地高辛标记寡核苷酸探针原位杂交技术，对新生期正常及甲减Wistar大鼠下丘脑Goα mRNA的表达状况进行研究。结果 (1)Goα mRNA主要分布于新生期Wistar大鼠下丘脑的弓状核(AR)、腹内侧核(VMH)等部位；(2)21日龄组大鼠下丘脑诸核团Goα mRNA的转录水平明显低于14日龄组；(3)甲状腺功能低下可引起14日及21日龄大鼠下丘脑AR、VMH核团Goα mRNA水平显著升高。结论 在大鼠下丘脑的发育过程中，信号蛋白Goα基因呈现特征性分布及表达，甲状腺激素对该基因具有转录下调作用。
【关键词】 甲状腺激素 G蛋白 原位杂交 大鼠
Effect of thyroid hormone on Go protein α subunit in hypothalamus of neonatal rat expression
CAI Dongsheng,LUO Min,CHEN Yuan,et al
Shanghai Institute of Endocrinology,Ruijin Hospital,Shanghai Seco?d Medical University,Shanghai,200025
【Abstract】 Objective To study the distribution of Go protein α subunit (Goα) mRNA in hypothalamus of neonatal rat and the effect of thyroid hormone on its expression. Methods Non-radioactive in situ hybridization technique was used to observe Goα mRNA distribution in the hypothalamic nuclei of 14- and 21-day Wistar rats, both normal and affected by thyroid hormone deficiency. Results (1) Goα mRNA was mainly located in arcuate nucleus (AR) and ventromedial hypothalamic nucleus (VMH) of 14- or 21-day rats; (2) The expression of Goα mRNA in the hypothalamic nuclei of 21-day rat was significantly weaker than that of 14-day rat; (3) Perinatal hypothyroidism could significantly increase Goα mRNA levels in AR and VMH of 14- and 21-day rats. Conclusion Goα mRNA is characteristically distributed and expressed in AR and VMH of neonatal rat, and the transcription of Goα gene in these nuclei can be down-regulated by thyroid hormone during development of rat brain.
【Key words】 Thyroid hormone G-protein In situ hybridization Rat
(Chin J Endocrinol Metab, 1999,15:286-289)
甲状腺激素在蔑发育过程中具有重要作用。通过大鼠模型可以发现，甲减鼠脑可表现为明显的形态结构改变和生化功能异常。近年来，有些学者开始从分子水平上对甲状腺激素调控脑发育的机制展开研究。目前緲经明确甲状腺激素可直接或间接调控多种脑结[1] [2] 下一页
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