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冠心病和脑梗塞患者载脂蛋白B基因多态性研究 |
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顾云 王绿娅 吴桂贤 王薇 刘静 秦兰平 刘军 吴兆苏
【摘要】 目的 观察apo B基因3′VNTR多态性在北京地区汉族人群、冠心病患者及脑梗塞患者中的分布特点，进一步探讨其与血脂变量的关系。方法 采用聚合酶链反应（PCR）扩增技术, 选择430例个体，其中冠心病组125例，脑梗塞组127例，健康对照组178例。结果 在430例个体中共分离到12个3′VNTR等位基因。对照组中以HVE32分布频率（41.0%）最高，冠心病组及脑梗塞组中以HVE34分布频率最高，分别为51.6%及55.9%, 冠心病组中串联重复拷贝数目大于等于40的等位基因分布频率高于对照组（P<0.05)， 同时冠心病组及脑梗塞组中大于等于42的等位基因也较对照组增多(P<0.05)。结论 apo B基因3′VNTR大等位基因的检出可能在一定程度上为揭示apo B基因在冠心病和脑梗塞脂质代谢中的作用提供了很好的遗传标记。
【关键词】 载脂蛋白B类 基因 冠状动脉疾病 脑梗塞
Polymorphism of the apolipoprotein B gene in patients with coronary heart disease and atherosclerotic cerebral infarction GU Yun, WANG Lüya, WU Guixian, et al. Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029
【Abstract】 Objective To investigate the polymorphism of variable number of tandem repeats (VNTR) 3 of the apolipprotein B (apo B) gene in patients with coronary heart disease (CHD) and atherosclerotic cerebral infarction (ACI) in Beijing area, and evaluate the association of polymorphism of 3′VNTR with level of blood lipid. Methods Using the polymerase chain reaction (PCR), the polymorphism of 3′VNTR was studied in 125 patients with CHD and 127 patients with ACI and 178 age-matched healthy individuals.Results Twelve segregating alleles of variable number of tandemly repeated loci of the 3′flanking region were observed. The most common allele was HVE32 in control group (41.01%) and HVE34 in CHD group (51.6%), ACI group (55.91%). Similarly, in CHD group and ACI group had frequency of 3′VNTR-B allele (number of repeat units≥42) was much higher than that in control group (P<0.05).Conclusion It is suggested that 3′VNTR allele might be a marker of heredity in the development of CHD and ACI presumably through their influences of lipid metabolism.
【Key words】 apolipoproteins B gene cardiovascular disease cerebral infarction
动脉粥样硬化性心脑血管病在我国已成为严重威胁人民健康的主要疾病。在形成动脉粥样硬化（athero[1] [2] 下一页
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