|
慢性乙型肝炎患者外周血T细胞受体基因重组的意义 |
| |
郭毅 吴辉 李十月 燕虹
【摘要】 目的 通过对慢性乙型肝炎患者外周血T细胞受体基因β链(T cell receptor gene Beta chain, TCRβ)重组与血清HBeAg，抗-HBe和丙氨酸转氨酶(ALT)平行检测，以探讨优势T细胞克隆在病程中的作用。方法 运用Southern杂交检测85例慢性乙型肝炎患者外周血单个核细胞DNA。结果 85例患者TCRβ重组阳性率为44.7%，大多数具有EcoRⅠ或/和HindⅢ重组带。横断面比较及对19例患者的随访表明：TCRβ基因重组的检出与HBV的清除和细胞毒作用有关。结论 TCRβ基因重组似可作为评价本病特异性免疫应答及肝脏损伤的一个指标。
【关键词】肝炎，乙型；T细胞受体基因；重组
Tcell receptor gene rearrangement of Peripheral blood mononuclear cells from patients with chronic he-patitis B
GUO Yi, WU Hui, LI Shiyue, et al.
(Department of Epidemiology, Department of Infectious Diseases, Hubei Medical University, Hubei Wuhan, 430071, China)
【Abstract】 Objective T cell receptor gene (TCR) rearrangement of peripheral blood mononuclear cells from patients with chronic hepatitis B was detected simultaneously with serum HBeAg, anti-HBe and alanine aminotransferase (ALT) to examine the role of dominant T cell clones. Methods Southern hybridization was used to detect DNA from peripheral blood mononuclear cells in 85 patients with chronic he-patitis B. Results The positive rate of TCR rearrangement was 44.7% in 85 patients with chronic hepatitis B. Most of them demonstrated two kinds of rearrangement bands of DNA fragment. Cross-sectional study indicated there was no significant difference in positive rate of HBeAg between TCR positive group and TCR negative group while there was significant difference in ALT between the two groups. Follow-up of 19 patients with chronic hepatitis B suggested that appearance of the TCR rearrangement was associated with cytotoxicity to liver and clearance of hepatitis B virus.Conclusion The detection of TCR rearrangement is useful in the evaluation of disease progression and immune response.
【Key words】Hepatitis B; T cell receptor gene; Rearrangement
乙型肝炎病毒(HBV)感染机体后可致不同的临床表现，其原因尚未明了。一般认为，T细胞对HBV的免疫应答决定HBV感染的结果［1］，慢性乙型肝炎的形成是由于细胞免疫不能彻底清除HBV所致。由于遗传因素决定T细胞受体(TCR)基因重组的方式，导致TCR[1] [2] 下一页
上一个医学论文： 卡氏肺孢子虫肺炎肺表面活性蛋白A和D的变化
下一个医学论文： 丙型肝炎病毒非结构蛋白3人源单链抗体的筛选与鉴定
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 内科论文 >> 正文