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上海地区 型丙型肝炎病毒包膜区cDNA序列分析 |
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邱纪慧 张欣欣 陆志檬 金根娣 汪垣
【摘要】 目的 分析上海地区丙型肝炎病毒(hepatitis C virus, HCV)Ⅱ、Ⅲ型包膜区cDNA序列变异。方法 采用逆转录-聚合酶链反应(RT-PCR)扩增HCV包膜区E1、E2/NS1片断(1 005bp)，在373DNA全自动测序仪上进行了序列分析。结果 HCV-E1、E2/NS1区核苷酸和氨基酸同源性比较显示：上海株Ⅱ型、Ⅲ型间同源性分别为62.48%和59.10%；上海株与国内外同型株间分别为85.97%～87.36%和83.38%～86.57%；上海株与国内外不同型株间分别为61.49%～70.95%和59.10%～78.21%。此外，共发现5个变异较大的区域，其中两个是国际公认的高变区(HVR1及HVR2)，另外3个为首次报道(第251～258、297～301、461～467位氨基酸)。结论 HCV-E1、E2/NS1序列变异大，这对其疫苗设计有参考价值。
【关键词】肝炎病毒,丙型；包膜区；核苷酸序列
Sequence analysis of E1,E2/NS1 region of genotype Ⅱ and Ⅲ hepatitis C virus isolated from Shanghai patients
QIU Jihui, ZHANG Xinxin, LU Zhimeng, et al.
（Research Unit of Clinical Virology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China）
【Abstract】 Objective To analyse the variation of envelope gene of genotype Ⅱ and Ⅲ hepatitis C virus (HCV) isolated from Shanghai patients with chronic hepatitis C. Methods The envelope genes (E1, E2/NS1) were amplified by nested reverse transcription polymerase chain reaction (RT-PCR). Totally 1 005 bp were sequenced and compared with previously reported isolates. Results The nucleotide (nt) and amino acid (aa) homologies between genotype Ⅱ and Ⅲ Shanghai isolates were 62.48% and 59.10%, respectively. The nt and aa homologies between Shanghai isolates and isolates with the same genotype from different regions were 85.97%～87.36% and 83.38%～86.57%, whereas the homologies between isolates with different genotypes, were 61.49%～70.95% and 59.10%～78.21%. Five hypervariable regions (aa 251～258, 297～301, 384～408, 461～467, 475～480) were found and two of them were consistent with the two hepervariable regions reported previously (aa 384～408, 474～482). Conclusion The variation of envelope gene was great and the knowledge of how variability distributed along different regions of the HCV genome could be helpful in the design of HCV vaccine.
【Key words】Hepatitis C virus; Envelope gene; Nucleotide [1] [2] [3] 下一页
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