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狼疮肾炎患者外周血单个核细胞内白细胞介素 |
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曾丽霞 孙林 叶任高 许韩师 陆才生 李幼姬 周飞宇 尹培达
【摘 要】 目的 了解狼疮肾炎(LN)患者外周血单个核细胞(PBMCs)白细胞介素-6(IL-6)与IL-6受体(gp80与gp130)的mRNA表达情况，并分析它们之间及其与自身抗体的关系。方法 采用逆转录聚合酶链反应(RT-PCR)方法检测18例活动期LN患者、16例非活动期LN患者和10名正常人PBMCs内IL-6、gp80与gp130的mRNA表达水平。结果 ①活动期LN患者、非活动期LN患者及正常人PBMCs IL-6 mRNA检出阳性率各为72.2%、37.5%和10.0%，前者显著高于后两者，而后两者PBMCs IL-6 mRNA的检出阳性率差异则无显著性；这三组人群PBMCs内gp80 mRNA与gp130 mRNA的检出阳性率均无统计学差异。②活动期LN患者PBMCs中IL-6 mRNA表达水平高于非活动期LN患者，后者又高于正常人；活动期LN患者PBMCs中有较高水平的gp80 mRNA与gp130 mRNA表达，而非活动期LN患者PBMCs gp80 mRNA与gp130 mRNA表达水平，与正常对照比较则无统计学差异。③活动期LN患者PBMCs内IL-6 mRNA、gp80 mRNA和gp130 mRNA相对表达水平互呈正相关，但在非活动期LN患者中则未见这种正相关关系。活动期LN患者PBMCs内IL-6、gp80和gp130基因表达水平与血清ANA、抗ds-DNA抗体和抗Sm抗体均无显著的相关性。结论 活动期LN患者PBMCs内IL-6及其受体gp80和gp130的mRNA表达均上调，且受体gp80和gp130的mRNA表达上调可能是其PBMCs内源性过多产生IL-6所致。
【关键词】 狼疮肾炎；白细胞，单核；白细胞介素6；gp80；gp130；聚合酶链反应，逆转录
Overexpression of interleukin-6,gp80 and gp130 genes in PBMCs from patients with active lupus nephritis
ZENG Lixia,SUN Lin,YE Rengao
(Institute of Kidney,First Affiliated Hospital,Sun Yat-sen University of Medical Sciences,Guangzhou 510080,China)
【Abstract】 Objective To clarify whether in vivo IL-6-IL-6 receptor system is up-regulated in patients with lupus nephritis (LN).The relationship between this system and serum autoantibody levels was also studied.Method IL-6 mRNA,gp80 mRNA and gp130 mRNA in freshly-isolated peripheral blood mononuclear cells (PBMCs) from 18 active and 16 inactive LN patients,as well as 10 healthy subjects were measured by quantitative reverse transcriptive polymerase chain reaction (RT-PCR).Results Significant levels of IL-6 mRNA were expressed in 13 (72.2%) of 18 active cases,whereas in 6 (37.5%) of 16 inactive cases and in 1 (10.0%) of 10 normal subjects.There was a higher positive rate of IL-6 mRNA in active cases than in inactive cases or normal subjects (P0.05 or P0.01).There were no differences in the positive rates of gp80 mRNA and gp130 mRNA by RT-PCR among the three groups (P0.05).[1] [2] [3] 下一页
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