VEGF和MVD在乳腺疾病血管生成中的意义

　　【关键词】 乳腺癌 VEGF MVD 免疫组化染色法

　　THE VALUE OF VEGF AND MVD IN BREAST

　　DISEASES ANGIOGENESIS

　　Wu Bianrong1, Li Jide2, Guo Zhenchang2, et al.

　　(1.Qinghai University Medical College;2.Qinghai University Affiliated Hospital)

　　Abstract Objective To detect the value of MVD and VEGF in breast disease angiogenesis. Methods The expression of VEGF and MVD in 70 cases of breast diseases was examined by immunohistochemistry staining (SP-method). Results VEGF expression and MVD value in breast cancer were much higher than benign breast disease, there was a correlation between VEGF expression and age, tumor size and HER2 in breast cancer and there was no correlation between other clinical parameters, but MVD was correlated with tumor size and HER2. MVD valve in VEGF high expression group was higher than VEGF low expression group (P <0.05). Conclusion It shows positive significance in detection of MVD and VEGF both in breast disease angiogenesis.

　　Key words Breast Cancer VEGF MVD Immunohistochemistry staining

　　乳腺癌与所有实体肿瘤一样, 其肿瘤细胞旺盛的代谢、增殖需要充足的血液供应, 其肿瘤新生血管形成与肿瘤的发生、发展密切相关。促血管形成因子的表达能促进肿瘤新生血管形成, 为肿瘤的生长、浸润及转移创造条件，血管内皮生长因子(VEGF)是已知作用最强、活性最高的促血管形成因子;微血管密度(MVD)是显示肿瘤血管形成活性的重要指标。我们分别应用抗Ⅷ因子抗体和VEGF单克隆抗体染色免疫组化方法检测乳腺疾病患者病例中MVD和VEGF表达情况, 旨在探讨其在乳腺疾病血管生成中的作用和意义。

　　1 材料与方法

　　1.1 临床资料

　　收集2006年～2007年于我院外科行手术治疗乳腺疾病病例70例;年龄32～64岁，中位年龄47岁;其中乳腺良性疾病患者28例，经病理证实的乳腺癌患者42例;乳腺癌组中无淋巴结转移者19例, 有淋巴结转移者23例;年龄小于45岁者16例，大于45岁者26例。早期(Ⅰ-Ⅱa期)病例16例，中晚期(Ⅱb-Ⅲ期)病例26例。因Ⅳ期患者未行手术治疗，故本资料中无Ⅳ期病例。

　　1. 2 主要试剂

　　第Ⅷ因子抗体、抗VEGF单克隆抗体及SP试剂盒购自福州迈新生物有限公司。

　　1. 3 染色方法

　　采用免疫组织化学染色法(S-P法

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