【摘要】目的 观察三甲益肝冲剂对大鼠肝纤维化的作用并探讨与抗脂质过氧化有关的作用机制。方法 雄性SD大鼠40只，随机分为模型组、复方丹参组、三甲益肝冲剂组和正常组。以二甲基亚硝胺(DMN)腹腔注射诱导大鼠肝纤维化模型，复方丹参组造模同时给予复方丹参灌胃，三甲益肝冲剂组造模同时给予三甲益肝冲剂灌胃，共4周。4周后处死大鼠取肝组织标本，光镜观察肝组织的病理变化，放射免疫法测定血清丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 与正常组比较，模型组肝组织Masson染色胶原纤维面积和血清MDA含量显著增加(P<0.05)，而血清SOD活性明显下降(P<0.05);与模型组比较，三甲益肝冲剂组Masson染色胶原纤维面积和血清MDA含量显著下降(P<0.05)，而SOD活性明显升高(P<0.05)。Masson 染色胶原纤维面积和血清MDA含量表达呈正相关关系(r=0.435, P<0.05)，与SOD活性呈负相关关系(r=-0.358, P<0.05)。结论 三甲益肝冲剂具有良好的抗实验性大鼠肝纤维化作用，其主要作用机制与抗肝脏脂质过氧化损伤有关。
【关键词】 肝纤维化;三甲益肝冲剂;肝星状细胞;脂质过氧化
Effects of Sanjia Yigan Granule on lipid peroxidation
in rats with hepatic fibrosis
Zhang Chaoxian, Qiao Hanchen, Yang Daokun, Qin Yongmei
(1. Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100; 2. Department of Infectious Diseases, the First Affiliated Hospital ofXinxiang Medical College, Weihui 453100, China)
ABSTRACT: Objective To observe the effects of Sanjia Yigan Granule (SJG) on hepatic fibrosis and explore its mechanism related to antilipid peroxidation in rats. Methods Forty male SD rats were randomly divided into four groups: normal group, model group, and compound Salviae Miltiorrhizae (Sm) group and SJG group. Dimethylnitrosamine was injected intraperitoneally for 4 weeks to induce hepatic fobrosis. At the same time of modeling, Sm and SJG were given in the corresponding groups. The rats were killed after four weeks. The histomorphylogic structure of the liver tissues was observed under optical microscope; the levels of MDA and SOD in serum were determined by radioimmunoassay. Results Compared with those in normal group, the collagen area in Masson staining and level of serum MDA were inreased obviously, and the level of serum SOD was dereased obviously in model group (P<0.05). Compared with those in model group, the collagen area and level of serum MDA were dereased obviously, and the level of ser
[1] [2] [3] [4] 下一页
您现在的位置： 绿色健康网 >> 医学论文 >> 内科论文 >> 正文