DNA含量及S期细胞比值与肺癌生物学特性的关系

　　摘要：目的 研究流式细胞术DNA含量及S期细胞比值(SPF)测定及其与肺癌临床病理指标的关系。方法 应用流式细胞仪(FCM)对56例肺癌新鲜组织及36例非癌性病变的肺新鲜组织的DNA含量(DI)和SPF进行测定，并结合临床病理资料分析其意义。结果 ①肺癌病变组织DNA含量、SPF均显著高于非癌性病变组织(P<0.01)。肺癌组异倍体率78.6%，而肺非癌性病变组异倍体率5.6%，两组相比差异有显著性(P<0.01);②肺癌组织DNA含量与肺癌组织学类型和临床分期无明显关系(P>0.05);③肺癌异倍体组SPF高于二倍体组(P<0.05)。肺癌SPF与肿瘤分期、转移呈正相关关系(P<0.05);随临床分期增加，SPF均相应增加(P<0.05)。结论 DNA含量改变、增殖活性的增加在肺癌组织中具有普遍意义。肺癌组织增殖活性越高，肿瘤转移的潜能越大。肺癌组织的FCM DNA含量及细胞增殖活性测定有助于进一步研究肺癌的生物学行为。

　　关键词：肺癌;流式细胞仪;DNA含量;生物学特征

　　Relation of FCM DNA content and Sphase fraction to the biological characteristics of lung cancer

　　Chen Juan, Zhang Jin

　　(Department of Respiratory, Affiliated Hospital of Ningxia Medical College, Yinchuan 750004, China)

　　ABSTRACT: Objective To investigate the relation of DNA content and SPF to the clinicopathological characteristic in lung cancer. Methods Fresh specimens taken from 56 patients with lung cancer and 36 patients with nonmalignant pulmonary lesions were measured for DNA index(DI), Sphase fraction (SPF) by using FACSCalibur 4200 flow cytometry. Results ① DNA index(DI) of lung cancer was 1.18±0.33, 0.99±0.07 in lung cancer and nonmalignant groups, respectively. The percentage of heteroploid was 78.6% in lung cancer, 5.6% in nonmalignant. DI and the positive rate of heteroploid were significantly higher in lung cancer groups than that in nonmalignant group(P<0.01); ② Athough DI increased as the pathological grade and TNM stage advanced, there were no significant difference(P>0.05); ③ It was demonstrated that SPF was significant higher in lung cancer groups than that in nonmalignant group. The SPF of heteroploid tumors was higher than that of diploid tumors(P<0.05); SPF correlated with TNM stage and lymph node and distant metastasis(P<0.05). With the increase of TNM stage, the SPF increased (P<0.05). Conclusion The increase of DNA content and proliferation ability improvement were common in lung cancer; With the proliferation

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