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慢性高原病患者骨髓组织Survivin 及VEGF表达水平和MVD变化 |
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【摘要】 目的 了解Survivin及VEGF在 慢性高原病(CMS)骨髓组织中的表达和MVD的变化，探讨细胞凋亡与血管新生在CMS发生、发展中的作用。方法 应用免疫组织化学SP法检测20例CMS患者(CMS组)及15例长期居住西宁地区健康人(对照组)骨髓组织中Survivin及VEGF的表达情况和MVD的变化(用CD34单抗标记的血管计数法)并进行比较分析。结果 CMS组Survivin及VEGF阳性表达率分别为70%、75%，均显著高于对照组(分别为26.67%及33.33%，P值均<0.05);CMS组MVD为31.93±6.13，也明显高于对照组(24.94±6.18)，P<0.01。CMS组Survivin阳性表达率、VEGF阳性表达率、MVD及Hb相互间均呈显著正相关(均为P<0.01)。结论 CMS骨髓组织中Survivin和VEGF表达升高, MVD增加，提示Survivin可能通过抑制细胞凋亡，与VEGF协同促进血管生成和红细胞增殖，参与CMS的发生发展。 袁霞(1976～)，女，汉族，青海籍，主治医师，硕士
【关键词】 凋亡抑制蛋白 血管内皮生长因子 微血管密度
THE STUDY ON THE EXPERSSIONS OF SURVIVINVEGF AND THE MVD IN BONE MARROW OF PATIENTSWITH CHRONIC MOUNTAIN SICKNESSYuan Xia1 ,Li Zhanquan1,Chai Kexia1, Ji Linhua1，Ye JunLin2,Guo XinJian2(1.Department of Hematology, Qinghai University Affiliated Hospital;2. Department of Pathology, Qinghai University Affiliated Hospital)Abstract Objective To explore the pathological roles of apoptosis and angiogenesis in the progenesis and progress of chronic mountain sickness(CMS) by evaluating the expressions of survivin and VEGF and the change of microvessel density(MVD) in bone marrow of patients with CMS. Methods The samples of bone marrow used in this study were obtained from 20 patients with CMS (CMS group) and 15 healthy adults (control group).The expressions of survivin and VEGF in bone marrows were detected using the SP immunohistochemistry technique and the MVD in bone marrow was measured by counting the vascular endothelial cells labeled by anti-CD34 monoclonal antibody. The results were analyzed comparatively .Results The positive rates of survivin and VEGF in CMS group were 70% and 75%,while 26.67% and 33.33% in control group. There were significant differences in the positive rates of survivin and VEGF between these two groups, respectively(P<0.05). MVD in CMS group (31.93±6.13) was significantly higher than that in control group (24.94±6.18 ) (P<0.01) .In the CMS group, there were positive correlations in the positive rates of survivi [1] [2] [3] [4] [5] 下一页
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