【摘要】 目的 检测人树突状细胞(DC)在 ACD保养液中不同时间的增殖和吞噬能力，为减少非溶血性输血反应提供实验依据。方法 抽取8例健康自愿献血者外周静脉血200 ml，ACD-B抗凝，4℃保存，在第1、2、3、4天分别取血20 ml，分离单个核细胞(PBMC)，孵育2 h洗涤后，贴壁细胞加入细胞因子联合诱导培养DC。灭活的酵母菌与DC以100∶1比例加入后37℃孵育1 h，取细胞悬液涂片，瑞-姬染色。光镜下计数吞噬酵母菌的DC，计算出DC的吞噬率和吞噬指数。结果 正常人外周静脉血第1天培养诱导的DC增殖生长良好，第2天仅有少量DC生长，第3、4天则无DC生长。第1天培养诱导的DC对酵母菌的吞噬率为78%～85%，平均值 81%，吞噬指数为 254。结论 在ACD保养液中的健康人的DC仅在离体后1～2天内能诱导增殖，诱导增殖后的DC具有较强的吞噬功能。DC 活性可能与某些非溶血性输血反应有关。
【关键词】 树突状细胞;ACD保养液;吞噬功能
Study on the changes of the dendritic cells in ACD preservative solution
YAO Rennan, CHEN Fuxing, LI Xi, WANG Zhende, LIU Junquan, ZHANG Zan, ZHAO Qin, SUN Fei
(Department of Blood Transfusion, The 97th Hospital of PLA, Xuzhou, Jiangsu 221004, China)
Abstract：Objective To provide experimental basis for clinical reduction of non-hemolytic transfusion reactions by detections of the proliferation and the phagocytic capacity of human dendritic cells (DC) in ACD preservation solution at different time points. Methods 200ml of peripheral vein blood was respectively collected from 8 healthy voluntary donors and was stored at 4℃, with anticoagulant ACD solution added. On days 1, 2, 3 and 4, 20 ml blood was respectively allocated to isolate peripheral blood mononuclear cells (PBMC). Having been incubated for 2 hours and rinsed, the adherent cells were supplemented with cell factors to induce DC culture. Inactivated yeast and the harvested DCs at the ratio of 100∶1 were incubated for 1 hour at 37℃, and Wright-Giemsa staining of the cell suspension smear was performed. The number of DCs that phagocytosed yeast was counted under the light microscope and the phagocytic rate and phagocytic index of DCs were calculated. Results The peripheral venous blood of normal donors of Day 1 had good proliferation of DCs and the blood of Day 2 had only a little growth of DCs, while the blood of days 3 and 4 had no DC growth. The phagocytosis rate of DCs induced and cultured on Day 1 was 78% ～ 85%, at an average of
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