【摘要】 目的 建立食管内脏高敏感性动物模型。方法 采用腹腔注射鸡卵清蛋白(OVA)基础致敏联合食管酸灌注的方法处理SD大鼠，并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。 结果 OVA基础致敏联合食管酸灌注组大鼠被激活了一个复杂而广泛的大脑网络，其在额顶皮质、岛叶、扣带皮质、中央杏仁核、Klliker-Fuse核、疑核、臂旁核、下丘脑室旁核、丘脑室旁核、三叉旁核、孤束核、最后区、延髓网状核等Fos样免疫活性(FLI)神经元的数目均显著高于其余各组(P<0.05)。模型组大鼠在中央杏仁核、臂旁核、室旁核、三叉旁核、孤束核的FLI阳性产物的平均光密度 (OD) 值亦较其余各组明显增高(P<0.05)。结论 预先腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注可成功建立食管内脏高敏感性动物模型。
【关键词】 内脏高敏感性;c-fos;免疫组织化学;食管酸灌注;卵清蛋白
[基金项目] 国家自然科学基金资助项目(30700362)
Establish and evaluation of the rat model of esophageal visceral hypersensitivity
YANG Min, CHEN Dong-feng, FANG Dian-chun
(Department of Gastroenterology, Daping Hospital, Third Military Medical University, Chongqing 400038, China)
Abstract: Objective To establish a reliable rat model of esophageal visceral hypersensitivity. Methods Healthy Sprague-Dawley (SD) rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-fos proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer. Results The basic ovalbumin-sensitized plus esophageal acid perfusion in model group initiated a high density expression of c-fos-immunoreactive (Fos-IR) neurons in multineuronal networks. A significantly higher number of Fos positive neurons was found in the model group than those in the corresponding regions of other groups(P<0.05)in the following brain areas: frontal and parietal cortex, insular cortex, cingulated cortex, central amygdaloid nucleus,the Klliker-Fuse nucleus, the nucleus ambiguus, parabrachial nucleus, hypothalamic paraventricular nucleus, paraventricular thalamic nucleus, paratrigeminal nucleus, the nucleus of solitary tract, area postrema, reticular nucleus of medulla,The OD values of Fos-IR neurons were also increased in the central amygdala , parabrachial nucleus, paraventricular nucleus, the paratrigeminal nucleus and NTS in the model group than th
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