大蒜素对胃上皮细胞Cx37 Cx43 mRNA表达及细胞间隙连接通讯功能的影响

【摘要】  　　目的 观察体外实验中大蒜素对胃上皮细胞系MGC-803 Cx 37 mRNA、Cx 43 mRNA表达及细胞间隙连接通讯功能的影响。方法 终浓度为3 μg/ml、6 μg/ml、9 μg/ml、12μg/ml大蒜素分别加入胃上皮细胞MGC-803培养 24 h、48 h、72 h，同时设立不加药物的阴性对照，用RT-PCR法检测细胞Cx 37 mRNA、Cx 43 mRNA表达，LY染料传输方法检测细胞间隙连接通讯功能。结果 MGC-803细胞Cx 37 mRNA有表达，Cx 43 mRNA无表达，GJIC功能弱，加入不同浓度大蒜素后，Cx 37 mRNA表达和细胞间隙连接通讯功能增强，Cx 37 mRNA表达随大蒜素浓度和培养时间的增加而增强（均P<0.05），Cx 43 mRNA仍无表达。 结论 大蒜素在转录水平上调胃上皮细胞MGC-803 Cx 37基因的表达，改善细胞间隙连接通讯功能。　

【关键词】  大蒜素；胃上皮细胞；细胞间隙连接通讯功能

　　Effects of allicin on expression of connexin 37，connexin 43 mRNA and gap junction intercellular communication of gastric epithelial cells

    LUO Xiao-Ling，XU Can-Xia，HU Lin.Department of Gastroenterology，The Third Xiangya Hospital of Central South University，Changsha 410013，China

    ［Abstract］  Objective  To observe the effects of allicin on the expression of connexin 37 mRNA，connexin 43 mRNA and gap junction intercellular communication of gastric epithelial cells MGC-803 in vitro.Methods  MGC-803 cell were interfered with various concentration allicin（3，6，9，12 μg/ml ） for 24，48 and 72 hours respectively and control groups with no intervention.RT-PCR method was applied to detect the expression of connexin 37 mRNA，connexin 43 mRNA in cells.Lucifer Yellow Scrape-loading and dye transfer method was applied to detect gap junction intercellular communication.Results  MGC-803 cell expressed Cx 37 mRNA，it didn’t express Cx 43 mRNA.Cell gap junction intercellular communication was weak in MGC-803 cell.The expression of Cx 37mRNA and gap junction intercellular communication was enhanced after interfered with variable dose allicin.The expression of Cx 37mRNA was up-regulated with the increasement of concentration and action time of allicin （P<0.05）.There was still no Cx 43 mRNA expression in MGC-803 cell.Conclusio

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