重庆地区HGV感染的分子流行病学

　　摘　要：目的探讨重庆地区HGV感染和基因型特征，了解致病性和传播途径。方法用RT-PCR和ELlSA方法检测685例献血员和76例血液透析患者HGV感染状况，比较肝功能和重叠感染情况并进行部分病例随访；进行HGV5-NCR测序。结果 本地存在HGV感染．血液透析患者HGV RNA阳性率(36％)明显高于献血员抗-HGV阳性率(3％)，约半数透析患者HGV合并HCV和HBV感染；基因分型表明属于第3组3b亚型。结论 hGV主要经血传播，感染HGV透析患者未发现有明显致病性；基因分型有助于深入探讨病毒致病性和变异。

Study on molecular epidemiology of hepatitis G virus infection in Chongqing

Jian Ping Liu ,Ya Fei Li(Department of Epidemiology,Third Military Medical University,Chongqing400038,China)

　　Abstract：AIM to understand the prevalence of HGV infection in Chongqing and mode of transmission as well as molecular biological characteristics of HGV.MIETHODS HGV rNA was detected by RT-PCR and anti-HGV antibody by ELISA. 5-NCR nucleotide sequences of some HGV RNA positive samples were determined and analyzed by molecular evolutionary methods. Four hemodialysis patients and one blood donor with HGV RNApositive were followed up.RESCLTS HGV RNA was positive in 36%(27/76) and anti-HGV in 20% (15/76) with hemodialysis patients, only3% (23/685) of anti-HGV in blood donors. Frequency of blood donating and plasmapheresis were risk factors for HGV infection in blood donors. Of HGV RNA positive patients, 13(48%) and 18 (67%) were observed to be co-infected with HCV and HBV respectively. HGV isolates from Chongqing belonged to 3b subtype of genotype3.CONCLUSION HGV infection may be spread through nosocomial infection besides blood transmission. Follow-up of one year to dialysis patients did not showpathogenicity of HGV.

　　Keywords：hepatitis g virus; hepatitis G/epidemiology; prevalence; sequence analysis;blood donor; hemodialysis

　　0引言庚型肝炎病毒(HGV)是近年来发现的与肝炎有关的新型人类病毒，属黄病毒科，为单股正链RNA病毒，基因组全长9．2kb～9．4kb，编码约2900个氨基酸残基的蛋白体．流行病学研究表明，HGV感染呈世界性分布，有关其致病性尚有争论．为了解重庆地区HGV感染情况，我们对献血员和血液透析患者进行了HGV感染的分子流行病学调查研究。1材料和方法1．1材料献血员685例为本校附属二院供血中心健康献血员，其中男541例，女144例，平均年龄30岁．血液透析患者76例为该院肾病中心血液透析室门诊和住院透析患者，其中男43例，女33例，平均年龄35岁．上述对象均为本地居住4a以上．标本采集：全血3mL分离血清，置-70°C保存，待检。1．2方法所有对象均行肝功能指标(全自动生化分析仪)、乙肝两对半、抗-HCV、抗-HGV(均用ELISA)检

[1] [2] [3] [4] 下一页