【摘要】 目的 探讨p53、bcl-2基因和增殖细胞核抗原(PCNA)等生物学 指标在胃癌前病变不同转归中的作用。 方法 采用免疫组化(LSAB)法 检测52例胃癌患者胃癌及癌前病变组织标本及56例非胃癌患者癌前病变〔胃黏膜中度以上肠 上皮化生和(或)异型增生〕组织标本的p53、bcl-2和PCNA的表达。 结果 (1)在癌前病变中，p53、bcl-2在癌组与非癌组之间的表达差异无 显著性(P＞0.05)，PCNA标记指数(PCNA LI)在癌组表达明显高于非癌组，差异有显著 性(P ＜0.01)；(2)p53、bcl-2和PCNA表达在胃癌组织均显著高于癌变前组织(P＜0.01)； (3)在肠型胃癌 ，癌前p53、bcl-2阳性者到癌时仍全为阳性，无1例转阴；在弥漫型胃癌，癌前bcl-2 阳性者到癌时有1例转阴。 结论 在肠型胃癌发生发展过程中，p53、bcl-2蛋白表达起重要 作用，且发生癌变前细胞增殖活性已明显升高。Expressions of p53, bcl-2 and proliferating cell nuclear antigen (PCNA) on diff erent transformation in precancerous lesions of the stomach
WU Yinqiao WANG Men gwei YOU Weidi, et al.
（Department of Gastroenterology, South Building, PLAGene ral Hospital, Beijing 100853,China）
【Abstract】 Objective To study the role of biological marks (p53, bcl-2 and PCN A) on different transformation in precancerous lesions of the stomach. Methods Immunohistochemical method (LSAB) was used to assess the expressions of p53, bcl-2 and PCNA in specimens from patients with (cancerous group, n=52) and wi tho ut (non cancerous group, n=56) gastric cancer. Results (1) In precancerous lesions, t here was no significant difference in p53 and bcl-2 expressions in cancerous gr o up and non-cancerous group (P＞0.05), PCNA expression in precancerous group was higher than that in non-cancerous group (P＜0.01). (2) p53, bcl-2 and PCNA expres sions in cancerous tissue were higher than those in precancerous group and non- cancerous group (P＜0.01). (3) In patients with intestinal-type carcinoma, p53 and bcl-2 remained expressed in precancerous tissue after developed into cancer, n o one was excluded. In diffuse-type carcinoma, bcl-2 expression changed into ne gative from positive in one patient. Conclusions The expression of p53 and bcl-2 significantly influenced the development of intestinal-type gastric carcinoma, a nd the proliferating activity had significantly increased before th
[1] [2] [3] [4] 下一页
您现在的位置： 绿色健康网 >> 医学论文 >> 内科论文 >> 正文