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131碘 |
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隋延仿 何凤昌 陈志南
【摘要】 目的 研究131Ⅰ标记肝癌单克隆抗体HAb18 Fab片段体内放射免疫显像效果。方法 以木瓜蛋白酶切割肝癌单克隆抗体HAb18制备HAb18 Fab,应用高效碘标记法将131Ⅰ标记于HAb18 Fab，再以131Ⅰ-HAb18 Fab偶合物进行荷肝癌裸鼠及肝癌病人体内放射免疫显像。结果 10只荷肝癌裸鼠全部明确显像，其中2只鼠4.5小时即出现瘤区放射性浓聚，8只鼠在8～12小时清晰显像,对照组鼠瘤区未出现放射性浓聚。131Ⅰ-HAb18 Fab的瘤/肝比值24小时为21.07±0.05，明显高于131Ⅰ-HAb18组最佳显像168小时的5.83±0.05。肝癌病人131Ⅰ-HAb18 Fab的最佳显像时间为16～24小时。结论 131Ⅰ-HAb18 Fab放免显像集中肝癌定位诊断、定性诊断和及时诊断三方面优点于一体，可能成为肝癌诊断的一项重要技术。
【关键词】 肝肿瘤 抗体，单克隆 放射性核素显像
Radioimmunoimaging of hepatocellular carcinoma with 131 Ⅰ labeled antihepatoma monoclonal antibody Fab fragment Sui Yangfang, He Fengchang, Chen Zhinan. Department of Pathology, Fourth Military Medical University, Xian 710032
【Abstract】 Objective To study the effects of radioimmunoimaging (R11) of hepatocellular carcinoma with 131Ⅰ labeled antihepatoma monoclonal antibody (HAb18) Fab fragment. Methods HAb18Fab was prepared by papain digesting HAb18, and it was radioiodinated with iodine-131 using high efficiency iodination method. The experiment consisted of two parts: ten nud mice bearing human hepatocellular carcinoma xenograft were injected intravenously with 131Ⅰ-HAb18Fab 3 700kBq/mouse, and six patients with hepatocellular carcinoma were injected intravenously with 131Ⅰ-HAb18Fab 111～166.5 mBq/body. Results Ten nud mice bearing hepatocellular carcinoma. showed positive image.The optimum imaging time was 4.5～12h after injection, and was 21.07±0.05 for tumor/liver higher than 5.83±0.05 of intact HAb18, and the reported 3.03. No tumor image was found in the control group of mice. Six patients with hepatocellular carcinoma, after injecting 131Ⅰ-HAb18Fab 16h，showed positive image. The optimum imaging time was 16～24h earlier than 96h of the reported 131 I Conclusion R11 with 131Ⅰ-HAb18Fab possesses the advantages of the location, qualitative analysis and earlier diagnosis. It is prospective in clinical application.
【Key words】 Hepatoma Monoclonal antibody[1] [2] 下一页
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