|
粘附分子P选择素单克隆抗体抑制胃癌转移的实验研究 |
| |
陈金联 章月星 储榆德 周 同
许蕙敏 程俊 李明烈
【摘要】 目的 研究P选择素单克隆抗体对SCID小鼠胃癌的抗转移作用。方法 用人胃癌组织块SGC-7901原位移植SCID小鼠制作转移模型。术后第3天开始，动物分别静脉注射P选择素单克隆抗体（单抗组，9只鼠），每次100 μg，每周2次，共3周；或PBS液体（PBS组，11只鼠），每次100 μl，每周2次，共3周。术后第6周末，所有动物处死，取肿大淋巴结和可疑转移脏器作病理学检查。结果 PBS组肿瘤转移（淋巴结、肝、肺等脏器）率为90.9％(10/11)，单抗组转移率为22.2％(2/9)。逆转录聚合酶链反应（RT-PCR）测定发现，SCID小鼠有癌转移者其胃癌组织中P选择素mRNA基因表达较不伴癌转移者明显增强，正常对照鼠胃组织P选择素mRNA表达水平较低。结论 P选择素及其基因mRNA表达与肿瘤转移相关，P选择素单克隆抗体具有抗肿瘤转移作用。
【关键词】 膜糖蛋白类 肿瘤转移 胃肿瘤
Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody Chen Jinlian*, Zhang Yuexing,Chu Yude, et al. * Shanghai Sixth Hospital,Shanghai 200233
【Abstract】 Objective To study the role of cell adhesion molecule P-selectin monoclonal antibody (Mab)in tumor metastasis of an orthotopic implantation metastatic model.Methods SCID mice were implanted orthotopically using SGC-7901 human stomach carcinoma intact tissue.Three days later,animals were given i.v.injections of PBS or P-selectin Mab (100μg/injection) twice weekly for 3 weeks.42 days after operation, all animals were sacrificed.Tissues from stomach and other organs were obtained for histopathological evaluation.Results Ten of the animals (n=11) treated with PBS developed metastatic tumors in the regional lymph nodes,liver,and lung. In contrast,2 of the animals (n=9)treated with P-selectin Mab developed metastatic tumors in the organs examined. The level of P、selectin mRNA expression determined by RT-PCR in gastric carcinoma tissue of SCID mice with tumor metastas is was higher than that without metastasis. Conclusions The expression of P-selectin adhesion moleculeis associated with tumor metastasis,and the metastasis can be arrested by the Mab.
【Key words】 Membrane glycoproteins Neoplasm metastasis Stomach neoplasms
(Natl Med J China, 1998, 78：437-439)
近年来，细胞粘附分子（如CD44,钙粘附蛋白等）在肿瘤转移过程中的作用已引起人们的重视。晚近发现的粘附分子受体P选择素，能介导内皮细胞和血小板[1] [2] 下一页
上一个医学论文： 放线菌酮对大鼠应激性溃疡的预防作用
下一个医学论文： 胚胎运动神经元种植至失神经骨骼肌内的观察
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 临床医学论文 >> 正文