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实验性过敏性脑脊髓炎中细胞因子的免疫组化研究 |
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于国平 朱克 田东华 周湘军
摘 要：本研究采用免疫组化方法对牛脊髓髓鞘碱性蛋白诱发的豚鼠实验性过敏性脑脊髓炎(EAE)中枢神经系统中IL-1β、TNF-α和IFN-γ的蛋白水平表达进行了研究。结果发现在EAE临床症状出现前3天，胸腰段脊髓中的血管周围浸润细胞和小胶质细胞样细胞开始表达这三种细胞因子，当临床症状出现时这种表达达高峰，并一直持续至临床高峰期。在恢复后期，活化的星形胶质细胞开始表达IL-β和TNF-α。它提示中枢神经系统中浸润细胞和小胶质细胞分泌的致炎性细胞因子如IL-1β、TNF-α和IFN-γ在EAE的发病中起作用。而在EAE的恢复期星形胶质细胞表达IL-1β可能与损伤的组织修复有关。
关键词：过敏性脑脊髓炎 白细胞介素-1β 肿瘤坏死因子-α 干扰素-γ
Study on IL-1β，TNF-α and IFN-γ in the CNS of experimental allergic
encephalomyelitis using immunohistochemistry
Yu Guoping
(Department of Neurology, Chinese PLA General Hospital, Beijing 100853)
Zhu Ke
(Department of Neurology, Chinese PLA General Hospital, Beijing 100853)
Tian Donghua, et al
(Department of Neurology, Chinese PLA General Hospital, Beijing 100853)
Abstract：Experimental allergic encephalomyelitis (EAE) is T cell mediated auto-immunological demyelinating disease and an animal model of multiple sclerosis. In present study, we induced EAE in guinea pigs using MBP and used immuohistochemical method to investigate the expression of IL-1β,TNF-α and IFN-γ in the central nervous system of EAE. The result showed that infiltrating cells and active microglia-like cells in the white matter of spinal cord expressed IL-1β, TNF-α and IFN-γ three days before the appearance of clinical symptoms. At the initial stage and the peak stage, this expression of cytokines became obviously and showed positive in the periventricular with matter of brain. At the recovery stage, this sxpression of cytokines decreased in infiltrating cells and microglia-like cells and astrocytes showed IL-1β and TNF-α positive. The results suggested that these proinflammatory cytokines participate in the pathogenesis and development on demyelinating diseases.
Key word：Allergic encephalomyelitis Interleukin-1β Tumor ne[1] [2] 下一页
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