【摘要】 目的 探讨负载SGC7901细胞抗原的DCCIK细胞对新城疫病毒(NDV)修饰SGC7901细胞的杀伤作用，探索胃癌新的治疗方法。方法 取健康成人外周血分离单个核细胞，用于诱导培养树突状细胞(DC)及细胞因子诱导的杀伤细胞(CIK细胞)。用反复冻融法制备胃癌细胞抗原，用于致敏DC细胞。然后分以下4组进行试验：CIK组、DCCIK组、CIK+ NDV组、DCCIK+NDV组，同时设单独的靶细胞对照(SGC7901)和效应细胞对照(CIK、NDV、DCCIK)。并与化疗药(氟尿嘧啶、顺铂)对SGC7901细胞的杀伤作用进行比较。结果 用NDV修饰抗原后，无论是CIK细胞还是DCCIK细胞对SGC7901细胞的杀伤率均增高。其中负载胃癌细胞抗原的DCCIK细胞杀伤率最高，效靶比为20∶1时，达到83.4%。化疗药物作用48 h时的疗效也不如单纯CIK细胞作用24 h的疗效。结论 负载SGC7901细胞抗原的DCCIK细胞对经NDV修饰抗原的SGC7901细胞的杀伤效果最佳。胃癌的生物治疗效果优于普通化疗。
【关键词】 胃肿瘤;抗原,肿瘤;免疫疗法;树突细胞;杀伤细胞;新城疫病毒
THE CYTOTOXIC EFFECT OF ANTIGENPULSED DCCIK CELLS ON NDVMODIFIED GASTRIC CARCINOMA CELLS LIU XICHUN, JIANG HAITAO, MAO WEIZHENG (Department of General Surgery, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China); [ABSTRACT] Objective To study the cytotoxic activity of SGC7901pulsed DCCIK cells on newcastle disease virus(NDV)modified SGC7901 cells, and explore a new therapeutic approach for gastric carcinoma. Methods Mononuclear cells were isolated from peripheral blood of healthy adults, and used for induction into dendritic cells (DC) and cytokine induced killer (CIK) cells. Gastric carcinoma antigen obtained through repeated freezing and thawing was used for pulsing DCs. The experiment included four experiment groups: CIK, DCCIK, CIK+NDV and DCCIK+NDV, and two control groups: target cell group(SGC7901) and effector cell group(CIK, NDV, DCCIK). The results were compared with the effects of chemotherapeutic agents (Fluorouracil, cisplatin) on SGC7901 cells. Results The cytotoxic activities of both CIK and DCCIK cells against SGC7901 cells were increased after antigen being modified by NDV. The cytotoxicity of DCCIK pulsed with gastric cancer cell antigen was the strongest (83.4%) with E∶T: 20∶1. The effect of chemotherapeutic agents, even after 48 hours of treatment, was less than that of CIK after 24 hours of treatment. Conclusion The cytotoxic activity of SGC7901pulsed DCCIK
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