【摘要】 目的： 探讨臭氧治疗对家兔骨性关节炎(OA)关节冲洗液中NO含量的影响。方法： 40 只新西兰兔随机分为5组，A组为正常对照组，B组为模型对照组，C、D、E组分别为臭氧(20、40、60 mg/L)治疗组;用Vandman法造模型成功后，将不同浓度的臭氧注射入C、D、E组兔膝关节腔内，每3天1次，连续2次;检测关节冲洗液中NO的含量，观察关节软骨的病理变化并进行病理评分。结果： B组软骨呈典型的OA改变，C、D组关节软骨可见不同程度修复，病理评分与B组相比有显著性差异(P<0.01或P<0.05);E组未见明显修复，病理评分与B组无显著差异;与A组相比，B组关节冲洗液中NO含量显著增高(P<0.01);与B组相比，C、D治疗组NO含量显著降低(P<0.01)，E组NO显著增高(P<0.01)。结论： 20～40 mg/L浓度的臭氧可促进实验性OA关节软骨修复，其机制可能与抑制NO生成有关，60 mg/L浓度的臭氧无软骨修复作用。
【关键词】 骨关节炎; 臭氧; 软骨，关节; 一氧化氮; 兔
The Impact of Ozone Treatment to Rabbits Osteoarthritis and NO Contents
WANG Lin, WANG Dashou, MU Qiong, YAO Jin, LU Wei, WO Chunxin
(Department of Pain Management, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004，Guizhou, China)
[Abstract] Objective: To explore the impact of ozone treatment to rabbit osteoarthritis (OA) and NO contents in joint flushing fluid (JFF). Methods： Forty New Zealand rabbits were randomly divided into five groups: normal control group (group A ), model control group (group B), and treatment groups C, D, and E which were treated with ozone at doses of 20 μg/ml, 40 μg/ml, and 60 μg/ml respectively. Models were established with Vandman method successfully, and different doses of ozone were injected into rabbit knee joint cavity of groups C, D, and E twice a week. NO contents in JFF and articular cartilage variation were determined and pathological changes in arthrodial cartilage were observed and analyzed. Results： Typical OA changes in cartilage were found in group B, while different degrees of repairing on articular cartilage could be seen in groups C and D of which the pathological scores differed from that of group B significantly (P<0.01,P<0.05), No obvious repairing was found in group E, of which the pathological score did not differ from that of group B. NO content in JFF was significantly higher in group B than in group A (P<0.01); NO contents in JFF of groups C and D were obviously lowe
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