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体外培养24小时受精失败卵母细胞单精子显微注射再授精的观察 |
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李蓉 庄广伦 周灿权 张敏芳
【摘要】 目的 探讨采用单精子显微注射(ICSI)技术处理体外培养24 h后常规体外受精(IVF)失败卵母细胞的效果。方法 对1997年6月至1998年10月在我院生殖医学研究中心接受常规IVF治疗的17例非男性因素不育患者,采用常规超排卵方案治疗,经阴道B超介导取卵, 共获卵179个, 体外培养24 h, 所有卵母细胞在常规IVF失败后,采用ICSI再授精。结果 共有130个卵母细胞常规IVF失败后进行ICSI再授精, 25个 (19.2%) 卵母细胞在注射过程中受到损伤死亡, 100个存活卵母细胞正常受精, 受精率为76.9%,共移植18个周期,2例临床妊娠。结论 如暂不考虑ICSI再授精对胚胎染色体的影响,对于体外培养24 h受精失败的患者,ICSI再授精不失为一个有效的补救方法。 【关键词】 卵母细胞;受精, 体外;胚泡移植
Reinsemination by Intracytoplasmic Sperm Injection 24-hour after Conventional in vitro Fertilization Failure
LI Rong,ZHUANG Guanglun, ZHOU Canquan,et al. (Department of Obstetrics and Gynecology, First Affiliated Hospital of Sun Yet-Sen University of Medical Sciences, Guangzhou 510080, China)
【Abstract】 Objective To examine the efficacy of intracytoplasmic sperm injection (ICSI) for rescue of conventional in vitro fertilization (IVF) failure. Methods From June 1997 to October 1998, 17 women with non-male factor infertility undergoing conventional ovarian hyper-stimulation protocol and IVF in our center, with fertilization failure were reinseminated by ICSI. Results Intracytoplasmic sperm injection of 130 unfertilized mature oocytes resulted in degeneration in 25 oocytes, successful fertilization occurred in 100 (76.9%) of the survival oocytes, and embryo transfer were done in 18 cycles. Two viable pregnancies resulted. Conclusion If chromosome abnormalities of embryos arising from reinsemination were ignored, our results suggest that ICSI is a successful means of rescuing conventional IVF failure. 【Key words】 Oocytes; Fertilization in vitro; Embryo transfer
1994年以来, 陆续有采用卵母细胞单精子显微注射(ICSI)技术处理体外培养24 h受精失败的卵母细胞, 并取得妊娠成功的报道[1]。我中心采用ICSI技术治疗17例体外培养24 h常规体外受精(IVF)失败、非男性因素不育患者,现将结果报告如下。
资料和方法
一、研究对象 为1997年6月至1998年10月,在本中心接受常规IVF治疗的17例非男性因素不育, 常规IVF取卵后24 h受精失败者。不育患者的不育原因包括输卵管阻塞、盆腔粘连、子宫内膜异位症、排卵障碍。女方平均年龄为(33.3±3.9)岁,男方平均年龄为(35.2±4.7)岁,不育时间为(6.9[1] [2] 下一页 上一个医学论文: 阴道超声照射对子宫内绒毛的影响 下一个医学论文: 妊娠高血压综合征患者血浆对体外培养的内皮细胞合成血管活性物质的影响
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