|
急性髓性白血病细胞CD87表达及其意义 |
| |
徐杰 柴忆欢 奚晓东 李祯萍
王津媛 白霞 王爱青 陆长明 阮长耿
【摘要】 目的 探讨小儿急性髓性白血病细胞尿激酶受体(uPAR, CD87)表达及其临床意义。方法 应用流式细胞术(FCM)检测了54例急性髓性白血病(AML)及20例急性淋巴细胞白血病(ALL)患儿CD87的表达，还用FCM及逆转录-聚合酶链反应(RT-PCR)法检测了U937、HL-60、K562细胞在氟波酯(PMA)作用下诱导分化过程中CD87表达的改变。结果 54例AML中33例（61%）CD87为阳性表达，20例ALL均为阴性表达。CD87阳性组与阴性组AML发生髓外浸润的比例分别为88%和19%(P<0.001)；外周血幼稚细胞百分率分别为（61.0±21.5）%和（30.5±15.2）%(P<0.001)；完全缓解（CR）率分别为52%和86%(P<0.05)；发生出血的比例分别为73%和62%(P>0.05)。U937、HL-60、K562经PMA诱导分化后，CD87表达增高。结论 CD87可作为白血病诊断、分型、判断预后的指标之一，也可作为诱导分化的指标之一。
【关键词】 受体，细胞表面 白血病，粒细胞，急性 流式细胞术
The expression and the clinical implication of CD87 on acute myeloid leukemia cells in children XU Jie, CHAI Yihuan, XI Xiaodong, et al. Children′s Hospital, Suzhou Medical College, Suzhou 215003
【Abstract】 Objective To explore the expression of receptor for urokinase (CD87) on acute myeloid leukemia (AML) cells in children and its clinical implication.Methods The expression of CD87 was detected with flow cytometry (FCM) in 54 AML patients and 20 acute lymphoblastic leukemia (ALL) patients. The expressionsa of CD87 on U 937, HL-60 and K562 cells were also studied with FCM and reverse transcription-polymerase chain reaction (RT-PCR) during differentiation induced by phorbol myristate acetate (PMA) in vitro.Results The staining for CD87 was positive in 33 of 54 patients with AML (61%) and negative in 20 patients with ALL. In CD87 positive and negative groups of AML, the percentages of infiltration of extramedullary tissues were 88% and 19% (P<0.001), respectively. The percentages of blast cells in peripheral white blood cells were（61.0±21.5）% and（30.5±15.2）% (P<0.001), respectively. The complete remission rates were 52% and 86% (P<0.05), respectively. The percentages of bleeding tendency were 73% and 62% (P>0.05), respectively. The CD87 expressions on U937, HL-60 and K562 cells were increasing during differentiation induced by PMA.Conclusion C[1] [2] 下一页
上一个医学论文： 儿童急性淋巴细胞白血病微量残留病细胞的DNA定量研究
下一个医学论文： 母乳喂养性黄疸及相关参数研究
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 妇产科论文 >> 正文