|
外源性Rb基因对卵巢癌细胞株生长的抑制作用 |
| |
李叶 张毅 李红霞 唐蔚清 闫春华 黎健
摘 要：目的 探讨外源性 Rb基因对卵巢癌细胞生长的抑制作用，为卵巢癌的基因治疗提供实验依据。方法 通过腺病毒介导的 Rb基因，于体外转染卵巢癌细胞株CAOV3。应用免疫组织化学、DNA琼脂糖电泳等方法，检测外源性 Rb基因的导入及其表达。通过四甲基偶氮唑蓝比色法及流式细胞术等，观察转染 Rb基因的CAOV3细胞的生长情况。结果 重组腺病毒载体可有效于体外转染卵巢癌细胞株CAOV3；转染外源性Rb基因后， CAOV3细胞内可检测到Rb基因及其蛋白表达；CAOV3细胞生长受到抑制，约68%的细胞生长停滞于G0期及G1期。结论 外源性Rb基因可通过腺病毒载体有效转染卵巢癌细胞株CAOV3，抑制CAOV3细胞的生长。
关键词：卵巢肿癌； 基因，视网膜母细胞瘤； 细胞系
Inhibition of Recombinant Rb Gene Adenovirus Vector on the Growth of Ovarian Cancer Cell Line
LI Ye ZHANG Yi LI Hongxia et al
（Department of Obstetrics and Gynecology, Beijing Hospital, Ministry of Public Health Beijing 100730, China）
Abstract：Objective To test and study the effect of inhibition on the growth of ovarian cancer cell line CAOV3 transfected by the recombinant Rb gene adenovirus vector. Methods The recombinant Rb gene and the LacZ gene in adenovirus vector were constructed and transfected into the CAOV3 cell line separately. The Rb gene expression was detected by immunohistochemical assay and agarose gel electrophoresis,etc. The inhibition on the growth of CAOV3 was tested by flow cytometry and methyl thiazolyl tetrazolium assay, etc. Results The constructed recombinant Rb gene adenovirus vector could infect cells with high level expression of Rb protein, and Rb cDNA could be found in the transfected cells. The proliferation of transfected CAOV3 cells was inhibited evidently. Sixty-eight per cent of cells were arrested at G0、G1 phases of cell cycle. Conclusions The recombinant Rb gene adenovirus vector could transform CAOV3 cells efficiently and inhibit CAOV3 cell growth. It might be a new approach of gene therapy for ovarian cancer.
Key words；Ovarian neoplasms; Genes, retinoblastoma; Cell line▲
外源性 Rb基因是与肿瘤发病相关的抑癌基因之一。许多肿瘤存在 Rb基因的缺失及失活。目前，国外已在不同癌细胞中进行了Rb基因的检测［1-4］，但尚未见Rb基因对卵巢癌细胞株作用的报道。本研究通过 Rb基因体外转染卵巢癌细胞株CAOV3，进行病毒转染及细胞生长的检测，以证实外源性 Rb基因对卵巢癌细胞生长的抑制作用。
材料与方法
一、 Rb基因重组腺病毒载体的构建
[1] [2] 下一页
上一个医学论文： 顺铂 维拉帕米和环孢菌素诱导卵巢癌细胞凋亡的研究
下一个医学论文： 子宫颈小细胞癌的临床及病理分析
|
|
|
|
|
|
|
您现在的位置： 绿色健康网 >> 医学论文 >> 妇产科论文 >> 正文