NS398通过转化生长因子 Smad信号通路对肾小球系膜细胞环氧化酶2表达的影响

【摘要】  目的：探讨COX-2抑制剂NS-398对转化生长因子-β（TGF-β）影响肾小球系膜细胞表达环氧化酶-2（COX-2）及Smad2 mRNA表达的作用。方法：传代培养系膜细胞后取第4代系膜细胞进行分组实验，用RT-PCR方法测定各组COX-2 mRNA及Smad2 mRNA的表达，观察不同浓度TGF-β在4、12、24 h对COX-2 mRNA及Smad2 mRNA表达的影响，及NS-398对其的抑制作用。结果：不同浓度TGF-β作用不同时间后，系膜细胞COX-2 mRNA和Smad2 mRNA表达均增加，呈浓度和时间依赖性。与TGF-β组相比，加入COX-2抑制剂NS-398后，COX-2 mRNA和Smad2 mRNA表达均减少。结论：TGF-β刺激肾小球系膜细胞COX-2表达的信号通路之一是Smad信号通路。而COX-2抑制剂NS-398对COX-2表达的抑制机制之一是通过此信号通路。

【关键词】  肾小球系膜细胞；环氧化酶-2抑制剂；转化生长因子-β；Smad2 ；环氧化酶-2；大鼠

 Effects of inhibitor of COX-2 on the expression of cyclooxygenase-2  by transforming growth

    factor β and Smad signaling  pathway in cultured rat mesangial cells

    CHEN Yulian , CHEN Xiaolan, LI Hui, et al      （1Department of Nephrology, the Affiliated Hospital of Nantong University,Nantong 226001， 2The Jiangsu Key Laboratory of Neuroregeneration）

    [Abstract]  Objective: To investigate the effects of inhibitor of COX-2 on the expression of cyclooxygenase-2  by transforming growth factor β and Smad signaling pathway in cultured rat mesangial cells.  Methods: Using the quart glomerular mesangial cell for experimentation, to observe the effect of different concentrations of TGF-β on the expression of COX-2 and Smad2 mRNA at 4, 12 and 24 hours, and the effect of the inhibitor of COX-2 NS-398 on the expression of COX-2 and Smad2 mRNA. The expression of COX-2 mRNA and  Smad2 mRNA was determined by RT-PCR. Results: After the glomerular mesangial cell was irritated by TGF-β, the expression of COX-2 mRNA and Smad2 mRNA increased. This depended on the density of TGF-β and the length of TGF-β acting time. After  NS-398（inhibitor of COX-2） was added, the expression of COX-2 mRNA and Smad2 mRNA reduced, which depended on the length of NS-398 acting time. Conclusion: One o

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