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子宫肉瘤组织DNA含量和细胞增殖水平的测定及临床意义 |
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谭晓嫦 陈耿生
【摘要】 目的 探讨子宫肉瘤组织的DNA含量及细胞增殖水平与临床病理特征及预后的关系。方法 应用流式细胞术测定75例石蜡包埋的子宫肉瘤组织中DNA含量及细胞增殖水平。结果 晚期、低分化的子宫肉瘤患者，其DNA异倍体率及DNA指数(DI)均明显高于早期、高分化患者（P<0.05）；晚期患者肉瘤组织中S期细胞比率(SPF)及增殖指数(PI)均明显高于早期患者（P<0.05）。DNA异倍体患者和二倍体患者的5年生存率分别为27%和76%，两者比较，差异有极显著性（P<0.01）；SPF>20%患者和SPF≤20%患者的5年生存率分别为10%和56%，两者比较，差异有极显著性（P<0.01）。结论 DNA异倍体及SPF、PI值高与子宫肉瘤的恶性生物学行为有关，DI、SPF、PI和临床分期是影响预后的最重要因素。
【关键词】 子宫肿瘤 肉瘤 DNA,肿瘤 S期
DNA Content and Cell Cycle Phase Analysis in Uterine Sarcomas and Its Clinical Significance
TAN Xiaochang*, CHEN Gengsheng.
*Maternal and Children health Care Hospital of Guangdong Province, Guangzhou 510120
【Abstract】 Objective To investigate the relationship among DNA content, cell cycle phase analysis with the clinical-pathological features and prognosis of uterine sarcomas. Methods DNA content and cell cycle phase analysis were performed on 75 paraffin-embedded specimens of uterine sarcomas using flow cytometry. ResultsBoth the aneuploidy rate and DNA index (DI) in specimens from advanced stage and poorly-differentiated cases were significantly higher than those in specimens from early stage and well-differentiated cases (P<0.05). S-phase fraction (SPF) and proliferation index (PI) were significantly higher in advanced stage than those in early stage(P<0.05). The 5-year suvival rates for DNA aneuploid and diploid patients were 27% and 76%, respectively (P<0.01). The 5-year suvival rates for patients with a SPF>20% and those with SPF≤20% were 10% and 56% respectively (P<0.01). Conclusion DI, SPF, PI and clinical stage are the most important factors affecting the prognosis.
【Key words】 Uterine neoplasms Sarcoma DNA,neoplasm S-phase
流式细胞术(Flow Cytometry, FCM)是70年代后期发展起来的一种自动快速定量分析细胞的新技术，已用于多种恶性肿瘤检测。但在妇科肿瘤的研究方面［1］，特别是子宫肉瘤方面的报道较少。本研究应用FCM，测定子宫肉瘤蜡块中肿瘤组织的DNA含量和细胞增殖水平，探讨DNA倍体、DNA指数(DI)及S期细胞比率(SPF)、增殖指数(PI)与子宫肉瘤的临床病理特征及预后的关系。现报道如下。
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