环氧合酶 2和ki 67在宫颈鳞状细胞癌组织中的表达

　　【摘要】 目的 研究环氧合酶-2(COX-2)和ki-67在慢性宫颈炎、宫颈上皮内瘤样变(CIN)、宫颈鳞状细胞癌(SCC)中的表达及其相关性。方法 RT-PCR法检测COX-2 mRNA在正常、炎性、CIN组织和SCC组织中的表达;免疫组化法检测各组中COX-2和ki-67蛋白的表达。结果 COX-2 mRNA和蛋白在正常组无表达，在炎性、CIN和SCC组表达升高(P<0.05)，COX-2蛋白的表达与肿瘤体积和浸润深度有关(P<0.05)。ki-67在正常组中偶有表达，在炎性组和CIN组表达增高，在SCC组显著增高(P<0.01)。COX-2与ki-67蛋白的表达相关(r=0.675，P<0.001)。结论 COX-2表达增高可能是宫颈癌发生发展的早期事件，与细胞异常增生有关。

　　【关键词】 环氧合酶-2;Ki-67;宫颈癌;细胞增殖

　　Expression of COX-2 and ki-67 in squamous carcinoma of the cervix

　　Zhao Xiumin, Wu Jing, Ma Yao

　　1. Department of Obstetrics and Gynecology, the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University, Xi’an 710004;

　　2. Department of Obstetrics

　　and Gynecology of the First People’s Hospital of Taizhou, 318020, China

　　ABSTRACT: Objective To study the expression of cyclooxygenase (COX-2) and ki-67 in chronic cervicitis, cervical intraepithelial neoplasia (CIN), squamous carcinoma of the cervix(SCC)and their relationship. Methods Tissue samples were harvested respectively from normal, inflammational, CIN and 30 SCC tissues. The expression of COX-2 mRNA was detected by RT-PCR method. COX-2 and ki-67 proteins were detected by inmmunohistochemical method. Results The overall expression of COX-2 mRNA and protein was higher in inflammation, CIN and SCC groups than that in normal cervix (P<0.05). COX-2 protein expression was correlated with tumor size and invasion depth (P<0.05). Ki-67 protein was higher in the other groups than that in normal cervix (P<0.05). Ki-67 was expressed in SCC group at a high level, which significantly differed from the expression in inflammation group (P<0.01). A significant correlation was found between COX-2 and ki-67 protein (r=0.675, P<0.001). Conclusion COX-2 may be an early incident of squamous cervical carcinogenesis related to cell proliferation.

　　KEY WORDS: cyclooxygenase-2; ki-67; cervical carcinoma; cell proliferation

　　环氧合酶-2(cycloox

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