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凋亡调控因子Survivin和Caspase |
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the differences among G3, G2 and G1 were significant (P<0.05). ② The positive rate of Caspase3 in normal endometrium, proliferative endometrium, and endometrial carcinoma was all 100% with no significant difference among them (P>0.05). However, the overexpression rate was 92.3%, 92.8%, and 60.0%, respectively. The differences among normal endometrium, proliferative endometrium and endometrial carcinoma were significant (P<0.05). In different endometrial adenocarcinomatous histological degrees, G1, G2 and G3, the overexpression rate of Capase3 showed the trend of decrease; however, there was no significant differences among the three groups (P>0.05). ③ The overexpression of Survivin and the expression of Caspase3 in endometrial carcinoma showed negative correlation (rs=0.287, P<0.05). Conclusion In endometrial carcinoma, the expression of suvivin increased, whereas the expression of Caspase3 decreased. The ability to suppress apoptosis enhanced, whereas the ability to promote apoptosis reduced. The balance between proliferation and apoptosis was broken, which might be one of the causes of developing endometrial carcinoma and related to its biological malignant behavior.
KEY WORDS: endometrial carcinoma; Survivin; Caspase3
子宫内膜癌是妇科临床常见的恶性肿瘤，发病率逐年上升。肿瘤分子生物学研究表明,细胞凋亡与许多疾病的发生有关,并且在肿瘤生长和侵袭中发挥重要作用。Survivin是凋亡抑制蛋白家族(inhibitor of apoptosis protein, IAP)新成员,参与细胞凋亡的调控;Caspase3是细胞凋亡的真正执行者。为了探讨凋亡调控因子Survivin和Caspase3在子宫内膜癌发生发展中的作用,我们采用免疫组化SP法对13例正常子宫内膜、28例增生性子宫内膜及50例子宫内膜癌中Survivin和Caspase3表达进行了研究。
1 材料与方法
1.1 研究对象 选取我院刮宫及手术切除标本的存档蜡块共91例，其中正常子宫内膜13例(增生期8例，分泌期5例)，增生性子宫内膜28例(单纯型增生13例，复杂型增生7例，不典型增生8例)，子宫内膜腺癌50例。所有子宫内膜癌均经病理证实，术前均未接受过放疗或化疗，所有病例在年龄等因素中组间有可比性(P>0.05)，按FIGO标准，子宫内膜腺癌细胞分化G1 23例，G2 17例，G3 10例。
1.2 实验方法 取常规固定、包埋的组织标本，5μm连续切片，分别用于HE和免疫组化染色。兔抗人Survivin多克隆抗体Sc10811购自北京中山生物技术有限公司，兔抗人Caspase3多克隆抗体BA0588购自博士德生物工程有限公司，工作浓度均为1∶50，SP试剂盒系Zymed 公司产品，实验步骤按试剂盒说明书进行，用PBS缓冲液代替第一抗体作为阴性对照。
1.3 结果判定 细胞质和(或)细胞核有棕黄色颗粒者为Sur 上一页 [1] [2] [3] [4] [5] 下一页
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