胎盘组织中Human AnnexinV的表达及与HBV感染的关系

　　摘要：目的 观察Human AnnexinV(HAV)在胎盘组织中的表达，并探讨其在乙型肝炎病毒(HBV)感染胎盘中的可能作用。方法 用免疫荧光染色法检测早孕绒毛，中孕和足月胎盘绒毛中HAV的表达;用免疫荧光双重染色法观察HBV感染的胎盘组织HBsAg和HAV的分布及相互关系。结果 ①所有的标本绒毛滋养层细胞、绒毛间质细胞、毛细血管内皮细胞都有HAV的存在。②HBV感染的胎盘绒毛中只要是HBsAg阳性的部位同时一定有HAV的表达，阳性信号存在于滋养层细胞、绒毛间质细胞、绒毛毛细血管内皮细胞中。结论 HAV可能作为HBsAg的特异性受体介导了HBV与胎盘组织各层细胞的粘附、内化等过程并最终导致了HBV对胎盘的感染。

　　关键词：胎盘;病毒受体;人膜联蛋白(HAV);乙型肝炎病毒(HBV)

　　Expression of Human Annexin V in placenta and its effects on placenta infected by HBV

　　Zhang Runju, Yue Yafei, Li Shuhong, Shi Ziyun, Ye Feng, Gui Qiaodi, Shi Lei

　　(Department of Obstetrics and Gynecology, First Affiliated Hospital, Medical School

　　of Xian Jiaotong University, Xian 710061; Department of Infections Diseases, First Affilited Hospital, Medical School of Xian Jiaotong University, Xian 710061, China)

　　ABSTRACT: Objective To investigate the expression of Human Annexin V(HAV) in placenta and to explore the possible role of it in HBV infected placenta. Methods HAV was detected in placentas that had been proved infected with HBV with immunofluorescence assays and the doublelabel immunofluorescence assays were used to detect HBsAg and HAV in the same placenta. Confocal laser scanning microscope was also used to observe the result. Results ① HAV was expressed on early period, metaphase and term villi. It was expressed on trophoblastic cell, interstitial cell and endothelium cell of interstitial blood vessels of villi. The result had no relation with the infection of HBV and the time of pregnancy. ② The doublelabel immunofluorescence assays showed the positive area of HBsAg in placentas accorded with the area of HAV. Conclusion HAV mediated HBV into cells of placenta as specific receptor and resulted in infection.

　　KEY WORDS: placenta; viral receptor; human annexin V(HAV); hepatitis B virus(HBV)

　　我国为乙肝高感染区，HBsAg携带者高达1.2亿，其中50%以上为母婴传播所致。而HBV宫内感染是母婴传播的重要途径，感染率可高达40%以上[1]。目前普遍认为HBV宫内感染主要经胎盘感染而致，即孕妇绒毛间隙血中的HBV感染绒毛滋养层细胞、 绒毛间质细胞、绒毛毛细血管内皮细胞后感

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