【摘要】 【目的】 观察骨形态发生蛋白-4(BMP-4)对人卵巢组织体外培养中始基卵泡存活和生长发育的影响。【方法】 34例卵巢组织切成2 mm × 2 mm × 1 mm皮质片，每例标本的皮质片均随机分成3组：研究组(BMP-4组)、对照组和非培养组，培养15 d后行组织学检查、雌孕激素测定和凋亡分析。【结果】 培养结束后对照组和研究组始基卵泡比例分别为58% ± 35%和48% ± 40%，低于非培养组(91% ± 9%)，P < 0.05;窦前卵泡比例分别为52% ± 40%和42% ± 35%，高于非培养组(9% ± 9%)，P < 0.05。研究组间质细胞凋亡指数和培养液孕激素浓度分别为(0.16 ± 0.08) μg/L和(3.6 ± 2.0) μg/L，低于对照组，对照组分别为(0.41 ± 0.16) μg/L和(6 ± 4) μg/L(P < 0.05)。【结论】 BMP-4可以促进始基卵泡向窦前卵泡转化，降低细胞凋亡率，抑制孕激素分泌，是卵泡生长发育过程中的促进因子。
【关键词】 卵巢组织; 体外培养; 始基卵泡; 骨形态发生蛋白-4; 卵泡发育
Effect of Bone Morphogenetic Protein-4 on Human Primordial Follicles in Vitro
SU Ning1， LI Yu2， WANG Wen-jun2， MAI Mei-qi2， YANG Dong-zi2， KANG Jia-li1，
ZHANG Qing-xue2
( 1. Department of Gynaecology and Obstetrics， Guangzhou First Municipal People′s Hospital， Guangzhou 510180， China; 2. The Center of Reproductive Medicine， The Second Affiliated Hospital， SUN Yat-sen University， Guangzhou 510120， China )
Abstract： 【Objective】 To evaluate the effect of bone morphogenetic protein-4 on the survival and development of human primordial follicles in vitro culture. 【Method】 Ovarian tissues from 34 patients were cut into pieces of approximately 2 mm × 2 mm × 1 mm， the pieces from the same patient were randomly distributed to parallel 3 groups， including study group (BMP-4 group)， control group， and non-culture group. Histological and apoptosis analysis as well as hormone measurement were performed after 15-day culture. 【Result】 In control group and study group， the percentage of primordial follicles were 58% ± 35% and 48% ± 40% after culture， significantly lower than that in the non-culture group (91% ± 9%)， P < 0.05. Meanwhile the percentage of preantral follicles in the study group and the control group was 52% ± 40% and 42% ± 35%， respectively， markedly higher than that in the non-culture group (9% ± 9%)， P < 0.05. The apoptotic index of ovarian interstitial cells and progesterone concentration of culture me
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